Primary Cell Line
Need primary cells for your assay development and research? We specialize in primary cell isolation, cell immortalization, iPSC generation and differentiation. Reach out to our team for tailored primary cell services.
Primary Cell Line Service
Primary cells are cells obtained directly from living tissues, preserving their natural morphology, growth traits, cellular markers, functions, and genetic stability. Patient-derived primary cells, specifically, are invaluable for exploring vital biological inquiries and facilitating high-throughput drug screening and subsequent multi-omic analyses.
At Acres Biosciences, we specialize in working with a diverse range of biospecimens for primary cell isolation, immortalization, iPSC services.
Primary cell isolation
Cell Types (Human Primary Cells): Immune cells: PBMCs, monocytes, T cells, B Cells; Pulmonary cells; Tumor cells.
Cell Types (Animal Primary Cells): Immune cells, spleen cells, brain cells, bronchial/tracheal cells, cardiac cells, prostate cells, pulmonary cells.
Species: Human, mouse, rat and some other animal.
Isolation Methods: Density gradient separation, magnetic isolation, mechanical isolation and enzyme digestion.
Deliverables: Ready-to-use primary cell lines, fully tested and authenticated to ensure purity.
Timeline: Varies based on project requirements. Please contact us for details.
Cell Immortalization Service
Cell Types: Fibroblasts, epithelial cells, neural cells, B cells, astrocyte, and more.
Species: Human primary cells.
Cell Immortalization Methods: We work with multiple immortalization genes, such as hTERT, SV40 T antigens, EBV, HPV-16 E6/7 gene, Adenovial E1A, CDK4, P53-siRNA, and cMyc. Note: hTERT cell immortalization and SV40T immortalization has been used for most cell types.
Deliverables: Immortalized cell lines, confirmed through mRNA and protein expression analyses, as well as functional assays or other requested assays.
Timeline: Varies based on project requirements. Please contact us for details.
iPSC Generation Service
Cell Types: Fibroblasts, PBMCs, LCLs
Species: Human, mouse, rat and some other animal.
Methods: Chemical or transgene reprogramming.
Deliverables: iPSCs. Our quality control validation includes morphology checks, AP staining, ICC staining for pluripotency markers, karyotyping and additional assays upon your request.
Timeline: Varies based on project requirements. Please contact us for details.
iPSC Differentiation Service
Cell Types: Neuron cells, microglia, muscle cells, etc.
Species: Human, mouse, rat and some other animal.
Methods: Specific factors tailored for your differentiation.
Deliverables: Cryovials of differentiated cells or live culture for cell types not suitable for cryopreservation. Customized QC based on your project requirements.
Timeline: Varies based on project requirements. Please contact us for details.
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