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ADC Assay Service
Acres Biosciences provides specialized in vitro ADC assays, including ADC internalization assay, cell cytotoxicity assays, and bystander effect analyses, to meet the specific needs of our clients in ADC validation.
Antibody-Drug Conjugates (ADCs)
Antibody drug conjugates (ADCs) are an innovative class of biological drugs that combine a potent toxin with a monoclonal antibody (mAb) through a linker, leveraging the antibody’s ability to target tumor cells and the toxin’s cell-killing power for complementary synergistic therapy. Recently, ADCs have shown tremendous therapeutic promise, revolutionizing cancer treatment.
At Acres Biosciences, we support the development of these groundbreaking therapies with expert ADC assay services, applying our extensive experience to meet your specific project needs and drive life-changing outcomes.
Ungaro, et al., Cells 2022, 11, 803.
ADC Assay Service
— 01
Antigen-antibody
binding assay
- Protein-based binding: direct ELISA, sandwich ELISA
- Cell-based binding: ELISA, flow cytometry (FACS), SPR (Surface Plasmon Resonance), etc.
- Before and after conjugation
— 02
ADC Internalization
assay
- pHrodo (Quick/high-throughput screening)
- Acid quench (Quick/high-throughput screening)
- Co-localization (Subcellular localization, MOA)
- Time-course method (Real-time, MOA)
— 03
Cytotoxicity
assay
- Regular ADC cytotoxicity assay: Assess ADCs with different linker-payloads or in different cells
- Bystander killing assay: Cytotoxicity of target negative/positive cell cocultures
- Immunogenic cell death (ICD) assay and apoptosis assay, etc.
— 04
Serum/plasma
stability assay
- Treatment: Incubate ADC in species-specific serum/plasma at 37°C.
- ELISA: Track total and conjugated antibodies to assess drug loss over time.
- LC-MS: Quantify free drug or drug-linker in serum/plasma samples.
— 05
Custom assay cell
engineering
- CRISPR knockout
- CRISPR knockin
- Overexpression
- Reporter cell line
- Primary cell line
- Learn More
— 06
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Service Highlight
Diverse Assay Formats
Distinct assay formats for thorough ADC evaluation.
Broad Cell Line Experience
Proven experience working with a wide range of cell lines
Extensive Experience
Successfully delivered 50+ projects.
US Operation
Robust US-based operations ensuring timely and efficient project execution.
Latest Blog Posts
Explore informative articles, tutorials, and thought leadership pieces covering a wide range of topics related to cell engineering, assay development, and biotech innovation.
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