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T Cell Engager Assay Service
Acres Biosciences provides end‑to‑end TDCC (T‑cell‑dependent cellular cytotoxicity) and T‑cell activation solutions—from quick feasibility screens to deeper mechanism‑of‑action and safety‑risk profiling.
T Cell Engagers (TCEs)
T-cell engagers (TCEs) are bispecific immunotherapeutics engineered to redirect endogenous T cells toward tumor cells by simultaneously binding a tumor-associated antigen and the CD3 receptor on T cells. This interaction forms an artificial immunological synapse that activates cytotoxic pathways and drives targeted tumor cell killing, independent of MHC restriction.
At Acres Biosciences, we advance the development of these next-generation therapies through specialized T-cell engager assay services, enabling precise measurement of cytotoxicity, T-cell activation, and cytokine release. Our scientifically rigorous and customizable platforms help de-risk candidate selection and accelerate translation from discovery to clinic.
Singh, et al., Br J Cancer 124, 1037–1048 (2021).
TCE Assay Service
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Dual binding assay
Evaluates TCE binding to both targets:
- Protein-based binding: ELISA
- Cell-based binding: ELISA, flow cytometry (FACS), SPR (Surface Plasmon Resonance), etc.
- Confirms simultaneous engagement of CD3⁺ T cells and target antigen–expressing cells
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T cell activation assay
Measures T-cell activation and functional response through:
- Activation markers: CD69 and CD25 expression by flow cytometry
- Cytokine release: IFN-γ, TNF-α, IL-2, etc. (ELISA or multiplex platforms such as MSD/Luminex)
- Proliferation: CFSE or CellTrace Violet dye dilution
- Degranulation: CD107a mobilization and granzyme B release indicating functional activation
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Cytotoxicity assay (TDCC)
Quantifies T-cell–mediated target cell killing through:
- Luciferase-based cytotoxicity assay (bioluminescence readout)
- Flow cytometry-based killing assay (e.g., 7-AAD, PI, Annexin V)
- LDH release assay for measuring target cell lysis
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Mixed Lymphocyte Reaction (MLR)
Evaluates non–antigen-specific T-cell activation using allogeneic PBMCs through:
- Cytokine release analysis to assess off-target activation and cytokine-release risk
- T-cell proliferation measurement for detecting non–antigen-dependent stimulation
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Custom assay cell
engineering
- Primary cell isolation (PBMC, T cells, NK cells, etc.)
- Reporter cell line
- CRISPR knockout/Knockin
- Overexpression
- Learn More
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Service Highlight
Diverse Assay Formats
Supporting comprehensive binding, activation, safety, and cytotoxicity studies.
Extensive Primary Cell Expertise
Deep expertise in PBMC- and T-cell–based functional assay development.
Proven Track Record
Successfully delivered 50+ projects.
US Operation
Robust US-based operations ensuring timely and efficient project execution.
Latest Blog Posts
Explore informative articles, tutorials, and thought leadership pieces covering a wide range of topics related to cell engineering, assay development, and biotech innovation.
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