Empowering Your Research
Cell-based assays
Our custom assay development services leverage cell biology to craft robust cell-based assays, providing invaluable insights into hit/lead efficacy, toxicity, and mechanism of action for your research.
Our Custom Assay Development Services
High-throughput Screening
Our high throughput screening services customize cell-based assays for efficiently analyzing candidate libraries. We integrate ELISA, flow cytometry, and luciferase reporter gene assays, expediting drug discovery.
Cell Viability and Proliferation
Assess cell viability, proliferation, and cytotoxicity using a variety of assay formats, including XTT and MTT cell viability assay, CellTiter Glo luminescent cell viability assay and more.
Measure gene expression, promoter activity, and cellular signaling pathways using reporter gene assays such as dual luciferase assay, GFP fluorescence assays, etc.
Potency Assays
Depending on the molecule’s mechanisms of action, we can design and conduct in vitro binding assay, enzymatic assay, Nab assay and other customized cell based potency assay.
Functional Assays
Evaluate cellular functions and activities through a variety of cell-based assays, including T cell functional assays (T cell activation assay, T cell proliferation assay, etc.), cell killing assay, cell invasion assay, cell migration assay and angiogenesis assay.
Primary Cell Assays
We excel in primary cell assays, with a focus on expert handling of various cell types such as PBMCs, T cells, B cells, macrophages, fibroblasts, tumor cells and more.
Expediate Your Biotech Research
Streamlined workflow For Custom Assay Development Services
01.
Initial Consultation
We begin by discussing the project requirements, objectives, and timelines with you to ensure a clear understanding of your assay development needs.
02.
Proposal Submission
Based on the initial consultation, we provide a detailed proposal outlining the scope of work, deliverables, timeline, and cost estimates.
03.
Project Kickoff
Once the proposal is approved, we initiate the project and assign a dedicated team to work closely with you.
04.
Regular Communication
Throughout the project, we maintain open communication with you, providing updates on progress and addressing any questions or concerns.
05.
Deliverables Submission
Upon completion of the project, we deliver the final results, reports, and any other deliverables to you for review and approval.
06.
Follow-up Support
We offer post-project support and assistance to ensure your satisfaction and address any additional needs or questions that may arise.
A Case Study: Cell-based Assay Development
Customer Requirements: Development of a cell-based assay for small compound screening
- To develop a robust high-throughput screening assay using cytokine ELISA.
- To conduct high-throughput screening utilizing their compound library.
Example Result of High-throughput Screening Services. Small molecule compounds were dispensed into a 96-well plate using a 3-fold series dilution from a top concentration of 1 µM by Tecan. Parental cells were then added to the compound plate, followed by a 2-day incubation period, after which supernatant was collected for cytokine ELISA assay. Left Panel: Representative standard curve of cytokine ELISA assay. Right Panel: High-throughput screening data using cytokine ELISA. The results were analyzed and plotted using Prism software to calculate IC50. Compound Q888 exhibited the highest potency, while compound M121 showed no activity.
Question?
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Visit our resource page to delve into our case studies, technical blogs, and ongoing promotions. Learn More
Our Assay Development Platforms
Our assay development services focus on customizing cell-based assays, utilizing our versatile platform designed to integrate cutting-edge techniques like flow cytometry, immunofluorescence, ELISA, and more for comprehensive analysis. This comprehensive approach enables us to analyze various aspects of cellular and molecular biology with precision and efficiency, empowering researchers to uncover valuable insights and drive scientific advancements.
Related solutions
Cell Line Engineering
Elevate your cell-based assay development with our tailored cell line engineering services. Whether it's CRISPR knockout or reporter cell lines, we specialize in stable cell line generation customized to meet your specific assay requirements.
Frequently Asked Questions
We stand out from other CROs. Here, you’ll find answers to common inquiries regarding our company and custom assay development services.
General Inquiry
San Diego, California.
Based in San Diego, California, we are a US-based company with a commitment to in-house operations for our cell line engineering and custom assay development services. When you collaborate with us, rest assured that your projects are managed entirely within the United States, setting us apart from competitors who may outsource their services internationally.
We offer a comprehensive range of services tailored to meet your research and development needs. Our services include:
- Stable Cell Line Engineering: Customized generation of stable cell lines expressing your gene of interest using various techniques such as lentivirus transduction, CRISPR/Cas9 gene editing, or traditional transfection methods.
- Custom Assay Development: Design and optimization of cell-based assays to evaluate compound efficacy, toxicity, and mechanism of action, including high-throughput screening assays, reporter gene assays, and functional assays.
We offer a multitude of advantages tailored to meet your needs.
- 50 Years of Experience: Providing solutions based on over five decades of collective industry experience.
- Cost Efficiency: Exceptional value coupled with unbeatable pricing in United States.
- Faster Data Delivery: Delivering cell lines in as few as 8 weeks, markedly exceeding the industry’s typical turnaround time.
- Confident Results: Implementing stringent quality control measures and validation protocols to ensure precise and reliable outcomes.
- 100% Customer Satisfaction: Your satisfaction is our top priority, and we guarantee responses within 24 hours to ensure your needs are promptly addressed and exceeded.
- US-based Company: Operating exclusively in the US, adhering to local regulations and standards, we eliminate international shipping for faster delivery, addressing concerns about lead times, cell line quality, and potential ethics issues.
Technology Platform
Cell-based assays are pivotal laboratory methodologies where cells are cultured ex vivo, permitting the meticulous examination of cellular responses to diverse stimuli. These assays serve as indispensable tools for elucidating intricate cellular behaviors, facilitating the rigorous evaluation of drug candidates for their efficacy and safety profiles.
The process of developing a cell-based assay typically involves several key steps:
- Define Objectives
- Cell Line Selection
- Optimize Cell Culture Conditions
- Assay Design and Protocol Development: Detection Method Selection
- Positive and Negative Controls
- Assay Validation: Optimization and Troubleshooting
- Documentation and Quality Control
- Validation and Application
Cell-based assays are required in various scenarios across biomedical research, drug discovery, and toxicology studies. Some common scenarios include:
- Drug Discovery and Development
- Target Identification and Validation
- Disease Modeling and Mechanistic Studies
- Toxicity Screening and Safety Assessment
- Pharmacological Profiling and Mechanism of Action Studies
- Functional Genomics and High-Throughput Screening
- Personalized Medicine and Precision Oncology
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