Hybridoma Sequencing Service
Experience the fastest and most accurate hybridoma sequencing available, enabling you to unlock the full potential of your antibody research.
Hybridoma Sequencing Service
At Acres Biosciences, we provide a comprehensive hybridoma sequencing service using both Sanger sequencing and NGS sequencing. From the moment you submit your hybridoma cell samples, our dedicated team ensures a seamless and personalized experience. Our comprehensive approach helps identify therapeutic leads for drug development, supporting your research goals effectively. Trust Acres Biosciences for superior sequencing results and dedicated service.
Sanger Sequencing
- Capabilities: Full-length antibody or variable regions of the antibody.
- Deliverables: Raw sequence data. Final sequence report with variable heavy chain and light chain or full-length DNA and amino acid sequence with CDR regions annotated.
- Advantages: When aiming to obtain the entire length of the antibody, the Sanger sequencing approach stands out for its exceptional advantages. By meticulously sequencing the complete antibody length, Sanger sequencing offers highly precise results, unveiling even subtle variations that could otherwise evade detection.
NGS Sequencing
- Capabilities: High-throughput sequencing.
- Deliverables: Frequency of the sequences identified by NGS; DNA and amino acid sequences, with CDR and FR regions annotated.
- Advantages: NGS technology is the preferred method for detecting low abundance variants, as it enables the identification of rare variants present at very low levels, crucial for applications requiring diverse sample identification.
Streamlined Workflow for Hybridoma Sequencing
01.
mRNA Extraction
- Mouse, rat, human, etc.
02.
cDNA Synthesis
- cDNA amplification
03.
Sequencing
- Sanger sequencing
- NGS sequencing
04.
Sequence Report
- Annotated report
- Original sequencing data upon request
05.
Delivery
- Fast turnaround
- Special offers available for bulk orders
Tips for sample preparation and shipping
Please submit hybridoma cell samples (>1×106 cells) and indicate the species and subtype of the monoclonal antibody. Besides hybridoma cells, we can also work with good quality RNA samples. Our project team will reach out and guide you on every steps of sample preparation and shipping.
Get In Touch with US
