Overexpression Stable Cell Line
Accelerate your discoveries with our custom overexpression stable cell lines, designed for exceptional performance and reliability.
Custom Overexpression Stable Cell Line Service
Gene overexpression is crucial for studying biological protein production and gene function. Overexpression cell lines are integral to various fields, including protein engineering, recombinant antibody development, and drug discovery. By integrating the gene of interest into host cells, we ensure permanent expression across generations.
At Acres Biosciences, we specialize in generating custom overexpression cell lines through advanced gene editing. Our services utilize both viral and non-viral vectors to achieve robust overexpression of genes of your interest, offering options for both constitutive or inducible expression of proteins or regulatory RNA.
Constitutive Overexpression Cell Line
Customization Options: Cell pool or single clone; single gene or multiple gene overexpression; adjustable expression levels (low, medium and high)
Cell Types: Various cell types, including both suspension and adhesion cells
Deliverables: Two overexpression cell pools or four overexpression single clones that tailored to your requested expression levels. (Note: Multiple expression levels are available upon request; additional fees may apply.)
Validation Method: Sequencing or Western Blot
Timeline: 8-15 weeks (cell pool), 14-20 weeks (single clone)
Inducible Overexpression Cell Line
Inducible Expression System: Tet-on and Tet-off system
Customization Options: Cell pool or single clone
Cell Types: Various cell types, including both suspension and adhesion cells
Deliverables: Two inducible overexpression cell pools or two overexpression single clones
Validation Method: Sequencing or Western Blot
Timeline: 12-24 weeks (cell pool), 18-30 weeks (single clone)
A case study: Stable Overexpression Cell Line
Customer Requirements: To establish stable cell lines with overexpression of either wild-type or mutant genes
Figure Legend: Generation and Validation of the Stable Overexpression Cell Line.
Panel A: Workflow for overexpression stable cell line generation: (1) constructing overexpression plasmids containing the gene of interest, (2) packaging these constructs into lentiviruses, (3) transfecting target cells with the lentivirus, (4) applying antibiotic selection to ensure only successfully transfected cells survive, forming a cell pool, and (5) using limited dilution to isolate and select single clones.
Panel B: Both wild-type and mutant gene overexpression were validated in selected single clones using Western Blot analysis, confirming the successful introduction and expression of these target proteins in the CRISPR knockout stable cell line.
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